Considerations To Know About high performance liquid chromatography
Considerations To Know About high performance liquid chromatography
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The solvent shipping system consists of a pump, through which solvent (cellular phase) is shipped at a controlled circulation price. If air receives dissolved during the cellular section, it may produce air bubbles that fluctuate the circulation price.
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- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
. Illustration of a standard high-performance liquid chromatograph with insets demonstrating the pumps that move the cellular period in the system as well as the plumbing utilized to inject the sample in the cell phase.
シリカゲルの粒子径が小さければ小さいほどピークの分離性は良くなるが、送液に必要なポンプの圧力が高くなる。そのため、ポンプ－インジェクター間、インジェクター－カラム間の配管の耐圧を上げたり、カラム自体を比較的高温の下にさらして溶媒の粘度を下げ、抵抗を小さくする工夫をしている。
各種の高速液体クロマトグラフィーの項目にある違いは、カラムの違いである事が多いため、装置はそのままでカラムの変更で行える場合が有る。ただし、誤って不適当な溶媒を通すとカラムを破損することがあるため、切り替えを行う際には注意が必要である。
測定時間は測定物質および測定パラメータによって大きく変動するが、一般的には数分から数十分/回程度である。
Therefore, most quantitative HPLC strategies don't need an interior regular and, rather, use more info exterior expectations and a standard calibration curve.
A pump forces a solvent by way of a column beneath high pressures of up to 400 atmospheres. The column packing product or adsorbent or stationary stage is often a granular content of good particles for instance silica or polymers.
Dimension-exclusion chromatography, often known as gel filtration or gel permeation chromatography, separates substances based upon their size and molecular bodyweight. Smaller molecules can penetrate the click here porous structure on the stationary stage and elute quicker, while larger sized molecules are held more time.
It's essential for laboratory personnel to achieve a basic comprehension of HPLC prior to making use of it to research compounds correctly and assure responsible effects.
Sample carryover: Sample elements can remain from the system soon after an injection, creating them to look in subsequent injections as ghost peaks. Assure proper rinsing on the injection system in between injections. Consider escalating the wash volume or using a more powerful wash solvent.
Movement price problems: Flow level specifically affects peak shape. A stream fee that is certainly also high can result in broader peaks due to significantly less interaction between analytes as well as stationary stage.